The EU directive requires ID to be sought either in entry or when gamblers purchase or sell chips to the value of Euro 1000 (about £600).
The rules will be particularly burdensome for e-casinos operating on the internet and certain forms of e-roulette which have become available online.
Computer systems will also be required to postively ID players.
The Gaming Board for Great Britain, in its annual report, said arrangements have been in place for many years covering the detection and reporting of money laundering activities.
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